Review



20x dna binding dye sample loading reagent  (fluidigm)


Bioz Verified Symbol fluidigm is a verified supplier
Bioz Manufacturer Symbol fluidigm manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    fluidigm 20x dna binding dye sample loading reagent
    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum <t>(20x),</t> and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.
    20x Dna Binding Dye Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20x+dna+binding+dye+sample+loading+reagent/DNA+Binding+Dye/pmc12540166-320-17-24
    Average 95 stars, based on 265 article reviews
    20x dna binding dye sample loading reagent - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Genetic reinstatement of RIG-I in chickens reveals insights into avian immune evolution and influenza interaction"

    Article Title: Genetic reinstatement of RIG-I in chickens reveals insights into avian immune evolution and influenza interaction

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1680791

    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.
    Figure Legend Snippet: H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.

    Techniques Used: Infection, Generated, Transgenic Assay, Expressing, Comparison, In Vivo

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Long-Term Engraftment of Cryopreserved Human Neurons for In Vivo Disease Modeling in Neurodegenerative Disease.
    Article Snippet: Samples were diluted 5-fold with DNA suspension buffer (Teknova). .. The qPCR was performed with Fluidigm’s 48.48 dynamic array IFC on the Biomark HD using 2X SsoFast EvaGreen Supermix with Low Rox (Bio-Rad Laboratories), 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), and primers at a final concentration of 500 nM. ..

    Article Title: Long-Term Engraftment of Cryopreserved Human Neurons for In Vivo Disease Modeling in Neurodegenerative Disease
    Article Snippet: Samples were diluted 5-fold with DNA suspension buffer (Teknova). .. The qPCR was performed with Fluidigm’s 48.48 dynamic array IFC on the Biomark HD using 2X SsoFast EvaGreen Supermix with Low Rox (Bio-Rad Laboratories), 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), and primers at a final concentration of 500 nM. ..

    Binding Assay:

    Article Title: Long-Term Engraftment of Cryopreserved Human Neurons for In Vivo Disease Modeling in Neurodegenerative Disease.
    Article Snippet: Samples were diluted 5-fold with DNA suspension buffer (Teknova). .. The qPCR was performed with Fluidigm’s 48.48 dynamic array IFC on the Biomark HD using 2X SsoFast EvaGreen Supermix with Low Rox (Bio-Rad Laboratories), 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), and primers at a final concentration of 500 nM. ..

    Article Title: FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155
    Article Snippet: For the assay mixes, 2.5 μL of 2X Assay Loading Reagent (Fluidigm), 2.3 μL of a primer pair mixture (with a final concentration of 1.15 μM), and 0.2 μL of low EDTA TE buffer were combined. .. For the sample mixes, 2.5 μL of TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μL of 20X EvaGreen DNA binding dye (Biotum), and 2 μL of the preamplified cDNA were mixed. ..

    Article Title: Exploring the Neuroprotective Effects of Grape Seed Procyanidins on Amyloid-β-Induced Toxicity in Caenorhabditis elegans
    Article Snippet: The pre-amplified cDNA samples were then analyzed by RT-qPCR on a BioMark HD system (Fluidigm, South San Francisco, CA, USA). .. For these assays, 2.25 μL of each amplified cDNA sample was combined with 2.5 μL of 2X SsoFast EvaGreen Supermix with Low ROX (Bio-Rad) and 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). .. RT-qPCR primer pairs (100 mM) were diluted 1:10 in Tris-EDTA (giving a total volume of 2.5 μL) and mixed with 2.5 μL Assay Loading Reagent (Fluidigm).

    Article Title: Intraarticular treatment with integrin α10β1-selected mesenchymal stem cells affects microRNA expression in experimental post-traumatic osteoarthritis in horses
    Article Snippet: .. Then a Pre-sample mix was made of 3 μL TaqMan Gene Expression Master mix (Applied Biosystems, Foster City, CA, United States), 0.3 μL 20X DNA Binding Dye Sample Loading Reagent (Fluidigm, San Francisco, CA, United States), 0.3μL 20 X EvaGreen (Biotium; VWR- Bie & Berntsen, Herlev Denmark), 0.9 μL low TE-buffer and 1.5 μL pre-amplified cDNA. .. Samples and primers were loaded in the microfluidic chip according to the manufacturer’s instructions, and the BioMark (Fluidigm, San Francisco, CA, United States) was used to perform the microfluidic qPCR cDNA technical triplicates, no template control, -RT samples, amplification curves, melting curves, and dilution curves all served as different quality controls points for each process and were used in the subsequent data preprocessing.

    Article Title: Neuroprotective Potential of the Flavonoids Quercetin and Epicatechin in a C. elegans Tauopathy Model
    Article Snippet: Finally, exonuclease I was added to eliminate unincorporated primers at 37°C for 30 min and 85°C for 15 min. Preamplified cDNA was then used for RT‐qPCR reactions completed in a BioMark HD System (Fluidigm, South San Francisco, CA, USA). .. A 2.25 μL aliquot of each amplified cDNA was mixed with 2.5 μL of 2X SsoFast EvaGreen Supermix with Low ROX (Bio‐Rad) and with 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). .. Individual RT‐qPCR primer pairs (100 μM) were diluted 1:10 with Tris‐EDTA (2.5 μL total volume) and mixed with 2.5 μL Assay Loading Reagent (Fluidigm).

    Article Title: Long-Term Engraftment of Cryopreserved Human Neurons for In Vivo Disease Modeling in Neurodegenerative Disease
    Article Snippet: Samples were diluted 5-fold with DNA suspension buffer (Teknova). .. The qPCR was performed with Fluidigm’s 48.48 dynamic array IFC on the Biomark HD using 2X SsoFast EvaGreen Supermix with Low Rox (Bio-Rad Laboratories), 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), and primers at a final concentration of 500 nM. ..

    Article Title: Exploring the Neuroprotective Effects of Grape Seed Procyanidins on Amyloid-β-Induced Toxicity in Caenorhabditis elegans
    Article Snippet: The pre-amplified cDNA samples were then analyzed by RT-qPCR on a BioMark HD system (Fluidigm, South San Francisco, CA, USA). .. For these assays, 2.25 μL of each amplified cDNA sample was combined with 2.5 μL of 2X SsoFast EvaGreen Supermix with Low ROX (Bio-Rad) and 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). .. RT-qPCR primer pairs Foods 2024, 13, 3865 6 of 17 (100 mM) were diluted 1:10 in Tris-EDTA (giving a total volume of 2.5 μL) and mixed with 2.5 μL Assay Loading Reagent (Fluidigm).

    Article Title: Genetic reinstatement of RIG-I in chickens reveals insights into avian immune evolution and influenza interaction
    Article Snippet: Quantitative PCR was performed in the BioMark HD instrument with the 96.96 IFC Dynamic Array (Fluidigm). .. The reaction was prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA. ..

    Concentration Assay:

    Article Title: Long-Term Engraftment of Cryopreserved Human Neurons for In Vivo Disease Modeling in Neurodegenerative Disease.
    Article Snippet: Samples were diluted 5-fold with DNA suspension buffer (Teknova). .. The qPCR was performed with Fluidigm’s 48.48 dynamic array IFC on the Biomark HD using 2X SsoFast EvaGreen Supermix with Low Rox (Bio-Rad Laboratories), 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), and primers at a final concentration of 500 nM. ..

    Article Title: Long-Term Engraftment of Cryopreserved Human Neurons for In Vivo Disease Modeling in Neurodegenerative Disease
    Article Snippet: Samples were diluted 5-fold with DNA suspension buffer (Teknova). .. The qPCR was performed with Fluidigm’s 48.48 dynamic array IFC on the Biomark HD using 2X SsoFast EvaGreen Supermix with Low Rox (Bio-Rad Laboratories), 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), and primers at a final concentration of 500 nM. ..

    Gene Expression:

    Article Title: FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155
    Article Snippet: For the assay mixes, 2.5 μL of 2X Assay Loading Reagent (Fluidigm), 2.3 μL of a primer pair mixture (with a final concentration of 1.15 μM), and 0.2 μL of low EDTA TE buffer were combined. .. For the sample mixes, 2.5 μL of TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μL of 20X EvaGreen DNA binding dye (Biotum), and 2 μL of the preamplified cDNA were mixed. ..

    Article Title: Intraarticular treatment with integrin α10β1-selected mesenchymal stem cells affects microRNA expression in experimental post-traumatic osteoarthritis in horses
    Article Snippet: .. Then a Pre-sample mix was made of 3 μL TaqMan Gene Expression Master mix (Applied Biosystems, Foster City, CA, United States), 0.3 μL 20X DNA Binding Dye Sample Loading Reagent (Fluidigm, San Francisco, CA, United States), 0.3μL 20 X EvaGreen (Biotium; VWR- Bie & Berntsen, Herlev Denmark), 0.9 μL low TE-buffer and 1.5 μL pre-amplified cDNA. .. Samples and primers were loaded in the microfluidic chip according to the manufacturer’s instructions, and the BioMark (Fluidigm, San Francisco, CA, United States) was used to perform the microfluidic qPCR cDNA technical triplicates, no template control, -RT samples, amplification curves, melting curves, and dilution curves all served as different quality controls points for each process and were used in the subsequent data preprocessing.

    Article Title: Genetic reinstatement of RIG-I in chickens reveals insights into avian immune evolution and influenza interaction
    Article Snippet: Quantitative PCR was performed in the BioMark HD instrument with the 96.96 IFC Dynamic Array (Fluidigm). .. The reaction was prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA. ..

    Amplification:

    Article Title: Exploring the Neuroprotective Effects of Grape Seed Procyanidins on Amyloid-β-Induced Toxicity in Caenorhabditis elegans
    Article Snippet: The pre-amplified cDNA samples were then analyzed by RT-qPCR on a BioMark HD system (Fluidigm, South San Francisco, CA, USA). .. For these assays, 2.25 μL of each amplified cDNA sample was combined with 2.5 μL of 2X SsoFast EvaGreen Supermix with Low ROX (Bio-Rad) and 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). .. RT-qPCR primer pairs (100 mM) were diluted 1:10 in Tris-EDTA (giving a total volume of 2.5 μL) and mixed with 2.5 μL Assay Loading Reagent (Fluidigm).

    Article Title: Neuroprotective Potential of the Flavonoids Quercetin and Epicatechin in a C. elegans Tauopathy Model
    Article Snippet: Finally, exonuclease I was added to eliminate unincorporated primers at 37°C for 30 min and 85°C for 15 min. Preamplified cDNA was then used for RT‐qPCR reactions completed in a BioMark HD System (Fluidigm, South San Francisco, CA, USA). .. A 2.25 μL aliquot of each amplified cDNA was mixed with 2.5 μL of 2X SsoFast EvaGreen Supermix with Low ROX (Bio‐Rad) and with 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). .. Individual RT‐qPCR primer pairs (100 μM) were diluted 1:10 with Tris‐EDTA (2.5 μL total volume) and mixed with 2.5 μL Assay Loading Reagent (Fluidigm).

    Article Title: Exploring the Neuroprotective Effects of Grape Seed Procyanidins on Amyloid-β-Induced Toxicity in Caenorhabditis elegans
    Article Snippet: The pre-amplified cDNA samples were then analyzed by RT-qPCR on a BioMark HD system (Fluidigm, South San Francisco, CA, USA). .. For these assays, 2.25 μL of each amplified cDNA sample was combined with 2.5 μL of 2X SsoFast EvaGreen Supermix with Low ROX (Bio-Rad) and 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). .. RT-qPCR primer pairs Foods 2024, 13, 3865 6 of 17 (100 mM) were diluted 1:10 in Tris-EDTA (giving a total volume of 2.5 μL) and mixed with 2.5 μL Assay Loading Reagent (Fluidigm).



    Similar Products

    95
    fluidigm 20x dna binding dye sample loading reagent
    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum <t>(20x),</t> and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.
    20x Dna Binding Dye Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20x+dna+binding+dye+sample+loading+reagent/DNA+Binding+Dye/pmc12540166-320-17-24
    Average 95 stars, based on 1 article reviews
    20x dna binding dye sample loading reagent - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.

    Journal: Frontiers in Immunology

    Article Title: Genetic reinstatement of RIG-I in chickens reveals insights into avian immune evolution and influenza interaction

    doi: 10.3389/fimmu.2025.1680791

    Figure Lengend Snippet: H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.

    Article Snippet: The reaction was prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA.

    Techniques: Infection, Generated, Transgenic Assay, Expressing, Comparison, In Vivo